بررسی مقاومت دارویی و فراوانی ژن‌های اینتگرون‌های کلاس I و II در انواع گونه‌های شیگلا جدا شده از بیماران مراجعه‌کننده به بیمارستان‌های شهر کرمان در سال 1393: یک گزارش کوتاه

Background and Objectives: The major mechanisms which contribute to the spread of shigellosis are antibiotic resistance and transmission from person to person. The main purpose of this study was determination of antibiotic susceptibilities and survey of the prevalence of integrons class I and II genes in Shigella species isolated from patients in Kerman hospitals Materials and Methods: In this cross-sectional study, 38 different Shigella species were collected from Kerman hospitals and were typed by serologically slide agglutination with specific antiesera. Antibiotic resistance patterns of shigella species were also evaluated by disk diffusion method. Detection of intI and intII genes was carried out by Polymerized Chain Reaction (PCR). Data analysis was done by Fisher,s exact test. Results: In the current study 21 (55.2%) S.flexneri, 14 (36.8%) S.sonnei, 3(7.8%) S.boydii and no S.dysenteriae were detected by serologically slide agglutination and the majority of strains were resistant to ampicillin but were sensitive to ciprofloxacin. Of all 38 Shigella isolates, 21 (55.2%) species were demonstrated intI gene but 32 (84.2%) species presented intII gene after performing PCR. Conclusion: According to high frequency of integrons and high resistance to antibiotics in shigella isolates, following health recommendations and prescribing correct antibiotics can prevent the extension of the diorrhetic disease resulted from shigella. Key words: Shigella, PCR, Integron, Antibiotic resistance, Disk diffusion, Kerman Funding: This research was funded by Kerman University of Medical Sciences. Conflict interest: None declared. Ethical approval: The Ethics Committee of Kerman University of Medical Sciences approved the study. How to cite this article: Abolhassanizadeh S, Mosadegh A, Moradi M, Hossieni Nave H, Taati Moghadam M. Determination of Antibiotic Susceptibilities and survey of the frequency of integrons class I and II genes in Shigella species isolated from patients in Kerman hospitals, Iran in 2014: A Short Report. J RafsanjanUniv Med Sci 2016; 14(11): 989-96. [Farsi]